抗生素溶液贮存液及工作浓度抗生素氨苄青霉素羧苄青霉素氯霉素卡那霉素链霉素四环素贮存液浓度 mg/ml贮存条件50（溶于水）-2050（溶于水）-2034（溶于乙醇）-2010（溶于水）-2010（溶于水）-205（溶于乙醇）-20工作浓度（ug/ml）严紧型质粒松弛型质粒2060206025170105010501050SDS-PAGE浓缩胶（5% Acrylamide）配方表组分H2O30% Acrylamide1.0M Tris-HCl（PH6.8）10%SDS10%过硫酸铵TEMED1ml0.680.170.130.010.010.0012ml1.40.330.250.020.020.002各种凝胶体积对应的各种组分取样量3ml4ml5ml2.12.73.40.50.670.830.380.50.630.030.040.050.030.040.050.0030.0040.005SDS-PAGE分离胶配方表组分名称6% GelH2O30% Acrylamide1.5M Tris-HCl PH8.810%SDS10%过硫酸铵TEMED8% GelH2O30% Acrylamide1.5M Tris-HCl PH8.810%SDS5ml2.611.30.050.050.0042.31.31.30.0510ml5.322.50.10.10.0084.62.72.50.1各种凝胶体积对应的组分取样量15ml20ml25ml7.933.80.150.150.0126.943.80.1510.50.20.20.0169.35.350.213.256.30.250.250.0211.66.76.30.2510%过硫酸铵TEMED10% GelH2O30% Acrylamide1.5M Tris-HCl PH8.810%SDS10%过硫酸铵TEMED12% GelH2O30% Acrylamide1.5M Tris-HCl PH8.810%SDS10%过硫酸铵TEMED15% GelH2O30% Acrylamide1.5M Tris-HCl PH8.810%SDS10%过硫酸铵TEMED0.050.0031.91.71.30.050.050.0021.621.30.050.050.0021.12.51.30.050.050.0020.10.003.32.50.10.10.0043.342.50.10.10.0042.352.50.10.10.0040.150.0095.953.80.150.150.00.963.80.150.150.0063.47.53.80.150.150.0060.20.0127.96.750.20.20.0086.6850.20.20.0084.61050.20.20.0080.250.0159.98.36.30.250.250.018.2106.30.250.250.015.712.56.30.250.250.01凝胶浓度与DNA长度浓度 %DNA长度 bp0.51000~300000.7800~120001500~100001.2400~70001.5200~3000250~2000e）配方表6ml4.110.750.060.060.0068ml5.51.310.080.080.00810ml6.81.71.250.10.10.01表
30ml15.967.50.30.30.02413.987.50.340ml21.28100.40.40.03218.510.7100.450ml26.51012.50.50.50.0423.213.312.50.50.30.01811.9107.50.30.30.0129.9127.50.30.30.0126.9157.50.30.30.0120.40.02415.913.3100.40.40.01613.216100.40.40.0169.220100.40.40.0160.50.0319.816.712.50.50.50.0216.52012.50.50.50.0211.52512.50.50.50.02
